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In isopycnic separations, particle density by yourself establishes ultimate position inside the density gradient. Figure twelve-9 compares the sedimentation coefficients and densities of a variety of mobile parts.
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It should be famous that not all markers are enzymes; DNA is an excellent marker of nuclei-wealthy fractions and chlorophyll is a wonderful chloroplast marker.
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These cons might not be really serious in some occasions, because the key submobile particles of a tissue homogenate have sedimentation coefficients that vary from one another by one or more orders of magnitude.
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